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Nuclear speckled pattern with striking variability in intensity with the strongest staining in g2 phase and weakest/negative staining in g1 Incorrect cytocentrifuge speed and time settings can accentuate these artifacts. The centromeres are positive only in prometaphase and metaphase, revealing multiple aligned small and faint dots.
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These patterns are a result of the adhesion of anas to nuclear antigenic structuresโthis is why they may be associated with certain autoantibodies and certain clinical conditions. The technique can stretch and distort cellular and nuclear morphology and allow nucleoli to appear more prominent than would normally be seen in peripheral smears In view of fast reactor analyses, it is shown that efficient nuclear data adjustments can be obtained on a limited assimilation database consisting of just six well documented integral parameters, i.e
